A spokeswoman for the 40-year-old TV personality said Thursday that Harrison and his wife are ending their 18-year marriage.
Harrison has hosted the ABC dating series and its various spin-offs since "The Bachelor" debuted in 2002.
"Though we have made this incredibly difficult decision to separate, our love and mutual respect remain, and we look forward to sharing in the lives of our wonderful children," the couple said in a joint statement.
They have two children together.
The eighth season of "The Bachelorette" is set to premiere May 14.