Palm Bay spokeswoman Yvonne Martinez said Sunday that the pilot, 65-year-old Paul Morrison of Melbourne, died over night at a hospital. His wife, 50-year-old Margo Morrison, died Sunday morning.
Martinez says the other passenger, 20-year-old Sasha Marger of Dallas, was released from the hospital Saturday.
Officials say Paul Morrison's plane crashed around 12:30 p.m. Saturday in a grassy field about 9 miles from the Valkaria Airport.
Several ATV riders alerted authorities to the crash and assisted the victims. Ernie Roper of Palm Bay tells Florida Today he saw the plane sputtering in the air before it plunged to the ground.
The National Transportation Safety Board is investigating.