"He said, 'I hope it wasn't venomous,' so I assured Robert I wouldn't actually let him play with venomous snakes," she added.
Terri Irwin said the couple's 9-year-old daughter, Bindi, was first bitten by a snake when she was 18 months old.
The girl, who is featured in the Discovery Kids Channel show "Bindi the Jungle Girl," posed for cameras with a new action figure in her likeness.
"It's every little girl's dream to have an exact look-alike doll. It's amazing," said Bindi, who was signing action figure toys of her late father.
Steve Irwin, known through his nature TV series as a wrangler of crocodiles and snakes, died in 2006 from a stingray's barbed tail during an underwater documentary shoot. He was 44.
Irwin provoked an international outcry in 2004 after being filmed holding his then 1-month-old son while feeding a snapping crocodile.