KSAT-TV reports that authorities from several agencies were called to a North Side neighborhood around 8:30 p.m. on Tuesday and found 55 people suspected of entering the country illegally in and around a tractor-trailer.
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Homeland Security Investigations spokeswoman Nina Pruneda confirmed that the agency is investigating.
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San Antonio Fire Department chief Charles Hood says all the people appeared to be in good health.
Hood said, "This truck was air-conditioned, thank God. This truck had water. So they were hydrated."
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Hood says five individuals were treated for minor injuries. They were transported to a hospital.
Except for the five injured, all of the trailer's occupants were taken to a detention center after they were searched.