The Wildcats (24-3) received 45 first-place votes from the 65-member national media panel on Monday to earn their third week on top of the poll. Kansas (23-4) was No. 1 on 20 ballots to hold second.
Villanova will have its toughest test yet as the top-ranked team when the Wildcats travel to No. 5 Xavier on Wednesday.
Oklahoma and Virginia are tied for third and are followed in the top 10 by Xavier, Michigan State, North Carolina, Iowa, Arizona and Maryland.
No. 21 Texas A&M and No. 22 Utah return to the rankings this week, replacing Dayton, which dropped out from 15th, and Providence, which was 23rd.
Bracketology: Virginia jumps into No. 1 seed discussion
ESPN bracketologist Joe Lunardi takes a look at the top line of his bracket and explains why Virginia has proved itself worthy of a No. 1 seed.
Hart's double-double leads Villanova past Butler
Josh Hart's double-double of 22 points and 12 rebounds fuels the Wildcats' 77-67 win over the Bulldogs.