The Oneida Indians say the record was set Saturday by 600 guests at the tribe's Turning Stone Resort Casino Event Center in central New York.
The 600 guests wore either a white or a red rain poncho and were told where to stand to form a human version of the ace of diamonds. Once in position, they had to remain in place for five minutes.
A Guinness World Records official was at Turning Stone to verify the record.
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