The Portland Press Herald reports the 65-year-old Struthers did not appear in York District Court on Thursday, and entered the plea through her lawyer.
Police arrested Struthers on Sept. 12 on U.S. Route 1 in the resort town Ogunquit. She was charged with criminal operating under the influence.
Struthers is best known for her role as Gloria Stivic in the 1970s TV sitcom "All in the Family." She had been performing at the Ogunquit Playhouse in the musical "9 to 5."
Struthers is scheduled to appear in court on Feb. 13 for a bench trial.