It happened at the InTown Suites on Highway 290 in north Houston just after 11 p.m.
Police said two Black men who were inside the room possibly got away in a car.
"When officers got there, there was quite a bit of narcotics. There was another pistol in the hotel room. So, they haven't really quite gotten to the bottom if it was just some kind of disturbance," Lt. R. Wilkens said.
The woman suffered a gunshot to the shoulder and was taken to a hospital for surgery, police said. She is expected to survive.
Police are looking for any surveillance video that may help identify the suspects. If you know any information regarding this incident, you are urged to call the Houston Police Department.