According to METRO, shortly before 2 a.m., the bus was heading northbound on I-45 near Edgebrook when the woman ran across the middle of the freeway.
She died.
The bus, which was headed to the METRO Polk Street bus operating facility at the time, was not in service.
No passengers were on board.
No details have been released about the woman's identity or age.
The crash shut down the freeway for a couple of hours, but has since reopened.
METRO police are investigating.