The woman, who wasn't named, is the first victim of the attack late Thursday in Cambrils, about 80 miles south of Barcelona.
It came hours after a van slammed into pedestrians on a busy Barcelona promenade, killing 13 people and injuring over 100 others.
In Cambrils, police shot dead five people wearing fake bomb belts who plowed into a group of tourists and residents with a car. In all, six people, including a police officer, were injured in the Cambrils incident.
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