The event started at 7:30 a.m. Sunday morning.
The 12th annual ride will begin and end at City Hall.
Mayor Turner told Houstonians that the riders will be heading to Missouri City for the first time in tour history.
2016 PHOTOS: Cyclists take to the streets in Tour de Houston
"I can't think of a better way for Houstonians to see our scenic city and enjoy the outdoors," said Mayor Turner.
Tour de Houston is part of the Mayor's Wellness Weekend which also includes the 40th Annual Bayou City Classic.
More details for riders and the event can be found at www.houstontx.gov/tourdehouston.