Veterinarians and SeaWorld staff spent three days giving Kyara critical care, but officials said her health continued to decline.
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Theme park officials said Kyara died early Monday morning from a likely case of pneumonia.
LOOKING BACK: Kyara last killer whale born in captivity at SeaWorld
Last killer whale born in captivity at Sea World in San Antonio.
A post-mortem examination has been scheduled to determine the calf's official cause of death.
"Kyara had a tremendous impact on each of her care staff, not to mention all of the guests that had the chance to see her," SeaWorld said, in a statement. "From late nights to early morning, rain or shine, we dedicate our lives to these animals, and this loss will be felt throughout the entire SeaWorld family."
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SeaWorld said it is monitoring the health of its other killer whales for any signs of illness.
Kyara was born in April to mom Takara.
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