The charges stem from an accusation of assaulting a woman in May. He has agreed to a peace bond.
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The Astros released a statement about the matter on Tuesday:
"Today, the Ontario Court of Justice withdrew the assault charge against Roberto Osuna. The Houston Astros look forward to Roberto continuing his commitment to be a productive and caring part of our community.
"The Astros remain committed to increase our support regarding the issues of domestic violence and abuse of any kind. We have engaged with a number of local, state and national organizations - and we look forward to working with them in the short term and over the long term."
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On May 8, Osuna was suspended when he was arrested for the domestic violence incident.
Osuna joined the Astros at the end of July in a trade that sent former Houston pitcher Ken Giles to the Toronto Blue Jays. On Aug. 4, the suspension expired, and he debuted for the Astros when he pitched a 1-2-3 inning vs. San Francisco on Aug. 6.
On Monday night, Osuna closed out the Astros' 5-3 win against his former club. The appearance, which was his first time playing in Toronto since the trade, was met with boos from Blue Jays fans.