The wreck happened around 11:15am in the 2600 block of Hamilton at McGowen.
According to officials, the ambulance was traveling westbound on McGowen when a truck on Hamilton failed to yield and struck the ambulance. The truck rolled over to its side and the driver had to be extricated from the cab. The ambulance sustained moderate damage.
Police say at least two people were taken to a nearby hospital, but none of the victims suffered life-threatening injuries.
An investigation into the cause of the wreck is ongoing.
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